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KMID : 1157520150110040251
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International Journal of Clinical Preventive Dentistry
2015 Volume.11 No. 4 p.251 ~ p.259
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Comparison of Streptococcus mutans Biofilm Formation on Dental Materials
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Cho Byeng-Ken
Kim Myung-Sook
Cho Ja-Won
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Abstract
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Objective: The authors had conducted this study for the purpose to compare the adhesion level of Streptococcus mutans to various oral appliance materials.
Methods: Six kinds of materials were used in this study. Bioplast, Sofliner Tough Medium, Duran, Ortho-Jet, Lucitone 199, Tokuyama Rebase II were selected. S. mutans ATCC 25175 was purchased from American Type Culture Collection and cultured with brain-heart infusion (BHI) broth. For biofilm formation, S. mutans was cultured in BHI broth supplemented 1% sucrose. S. mutans was mixed BHI broth including 2% sucrose after cultivation for overnight. And, formation and observation of whole bacteria and S. mutans in salivary biofilm on specimens, and measurement of saliva protein on specimen were carried out.
Results: The adhesion level of S. mutans and whole bacteria is lower in resilient materials than in rigid materials. Sportguard wafer is the lowest adhesion level of S. mutans and whole bacteria among the resilient materials. There were no differences of adhesion level of S. mutans and whole bacteria among the rigid materials (p＜0.05). Saliva protein was more attached on rigid materials than on resilient materials.
Conclusion: It was recommended to clean the oral appliance periodically.
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KEYWORD
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Streptococcus mutans, biofilms, saliva proteins
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